NLM IRP Seminar Schedule
UPCOMING SEMINARS
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May 7, 2024 OPEN
TBD -
May 9, 2024 Pascal Mutz
The Riboviria protein structurome expands virus protein annotation and highlights protein relations -
May 14, 2024 Stanley Liang
TBD -
May 16, 2024 Diego Salazar
TBD -
May 21, 2024 Ziynet Kesimoglu
TBD
RECENT SEMINARS
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May 2, 2024 OPEN
TBD -
April 30, 2024 Wenya Rowe
The conformal central charge of the spin-1/2 XX model derived from long-chain asymptotics -
April 25, 2024 Ermin Hodzic
Condition-Aware Cell Type Deconvolution of Bulk Tissues -
April 16, 2024 Jaya Srivastava
Regulatory plasticity of the human genome -
April 11, 2024 Sergey Shmakov
Comprehensive survey of the TnpB RNA-guided nucleases
Scheduled Seminars on May 5, 2022
Contact NLM_IRP_Seminar_Scheduling@mail.nih.gov with questions about this seminar.
Abstract:
Insertion sequences of IS200/IS605 and IS607 usually contain only the genes that are required for their transposition and its regulation. These elements encode tnpA transposase, which is essential for mobilization, and often carry an accessory tnpB gene. Previous studies showed that TnpB might be a predecessor of the CRISPR–Cas9/Cas12 nucleases, and recently shown to be a RNA-directed nuclease that is guided by an RNA. In our work we provide a pipeline for systematic identification and characterization for TnpB genes. We obtain information of TnpB mobility obtained from completely sequenced genomes. We use phylogeny based methods to identify novel conserved TnpB families using their mobility and propose their novel function. This study expands our understanding of TnpB diversity and its applicability in genome editing.